Biomaterial Database

[Carboxylic proteinase from the basidiomycete Russula decolorans Fr 0456]. 1980

G N Rudenskaia, and A V Gaida, and V M Stepanov

A carboxylic proteinase has been isolated from a cultural filtrate of the basidiomycet Russula decolorans Fr 0456. A sequential chromatography on biospecific adsorbents containing polypeptide antibiotics gramicidin S and bacitracin used as ligands resulted in a 186-fold purification and 48% yield of the enzyme. The molecular weight of the enzyme is 35 000, pH-optimum of hemoglobin hydrolysis is 2,2, the isoelectric point lies at 4,9. The enzyme revealed a pronounced milk-clotting activity and is completely inactivated by the specific inhibitors of carboxylic proteinases--N-diazo-acetyl-N'-2,4-dinitrophenylethylenediamine and pepstatin. Its amino acid composition was also found to be similar to that of the carboxylic proteinases isolated from microscopic fungi. The data obtained suggest that the carboxylic proteinase from the basidiomycet Russula decolorans Fr 0456 is closely related to pepsin-like carboxylic proteinases, particularly to those produced by microscopic fungi.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010434	Pepsin A	Formed from pig pepsinogen by cleavage of one peptide bond. The enzyme is a single polypeptide chain and is inhibited by methyl 2-diaazoacetamidohexanoate. It cleaves peptides preferentially at the carbonyl linkages of phenylalanine or leucine and acts as the principal digestive enzyme of gastric juice.	Pepsin,Pepsin 1,Pepsin 3
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D001487	Basidiomycota	A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.	Basidiomycetes,Basidiomycete,Basidiomycotas