Biomaterial Database

A rapid and simple gas-liquid chromatographic determination of valproic acid (alpha-propyl-valeric acid) in serum. 1980

M Puukka, and R Puukka, and M Reunanen

A simple and rapid gas-liquid chromatographic method for the determination of valproic acid in serum is described. Valproic acid is extracted from acidified serum into chloroform and analyzed by gas-liquid chromatography using an FFAP column. The sensitivity of the method is 5 mumol/l and the within-run and day-to-day precisions (CV) are 4.5% or better. Analytical recovery is estimated to exceed 98% and no interference from other drugs or constituents of serum has been observed. The method was used to study the half-life of valproic acid in five healthy persons receiving a single oral dose of the drug, and the technique is well adapted to the routine monitoring of epileptic patients. In routine analysis, the determination of 50 serum samples may be performed by one technician during a normal working day.

UI	MeSH Term	Description	Entries
D004827	Epilepsy	A disorder characterized by recurrent episodes of paroxysmal brain dysfunction due to a sudden, disorderly, and excessive neuronal discharge. Epilepsy classification systems are generally based upon: (1) clinical features of the seizure episodes (e.g., motor seizure), (2) etiology (e.g., post-traumatic), (3) anatomic site of seizure origin (e.g., frontal lobe seizure), (4) tendency to spread to other structures in the brain, and (5) temporal patterns (e.g., nocturnal epilepsy). (From Adams et al., Principles of Neurology, 6th ed, p313)	Aura,Awakening Epilepsy,Seizure Disorder,Epilepsy, Cryptogenic,Auras,Cryptogenic Epilepsies,Cryptogenic Epilepsy,Epilepsies,Epilepsies, Cryptogenic,Epilepsy, Awakening,Seizure Disorders
D005410	Flame Ionization	Pyrolysis of organic compounds at the temperature of a hydrogen-air flame to produce ionic intermediates which can be collected and the resulting ion current measured by gas chromatography.	Flame Ionizations,Ionization, Flame,Ionizations, Flame
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014635	Valproic Acid	A fatty acid with anticonvulsant and anti-manic properties that is used in the treatment of EPILEPSY and BIPOLAR DISORDER. The mechanisms of its therapeutic actions are not well understood. It may act by increasing GAMMA-AMINOBUTYRIC ACID levels in the brain or by altering the properties of VOLTAGE-GATED SODIUM CHANNELS.	Dipropyl Acetate,Divalproex,Sodium Valproate,2-Propylpentanoic Acid,Calcium Valproate,Convulsofin,Depakene,Depakine,Depakote,Divalproex Sodium,Ergenyl,Magnesium Valproate,Propylisopropylacetic Acid,Semisodium Valproate,Valproate,Valproate Calcium,Valproate Sodium,Valproic Acid, Sodium Salt (2:1),Vupral,2 Propylpentanoic Acid