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Glutaraldehyde-fixed toad urinary bladders were incubated with the sterol specific antibiotic, filipin, and the distribution of the resulting filipin-sterol complexes was studied in freeze-fracturing replicas. While the luminal membrane of some cells had a heavy, homogeneous labelling, most had clusters of filipin-sterol complexes scattered on an otherwise poorly labelled membrane. These clusters were seen to overlie cytoplasmic granules, which are found just beneath the luminal plasma membrane of this cell type, and which were always heavily labelled. These results suggest that (a) the granule membranes have a high cholesterol content and (b) sites of interaction between granule and luminal membranes might be enriched in cholesterol. In addition, filipin-sterol complexes were absent from vasopressin-induced particle aggregates on the granular cell luminal membrane.
L Orci, and
R Montesano, and
D Brown
February 1982,
Brain research,
L Orci, and
R Montesano, and
D Brown
September 1979,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
L Orci, and
R Montesano, and
D Brown
May 1987,
The Journal of protozoology,
L Orci, and
R Montesano, and
D Brown
December 1981,
Biochimica et biophysica acta,
L Orci, and
R Montesano, and
D Brown
December 1984,
The Journal of comparative neurology,
L Orci, and
R Montesano, and
D Brown
January 1992,
Andrologia,
L Orci, and
R Montesano, and
D Brown
June 1984,
Laboratory investigation; a journal of technical methods and pathology,
L Orci, and
R Montesano, and
D Brown
January 1984,
Histochemistry,
L Orci, and
R Montesano, and
D Brown
December 1984,
Experimental parasitology,
L Orci, and
R Montesano, and
D Brown
August 1978,
The Journal of cell biology,
