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Glucose-induced inhibition of Ca(++) extrusion from the beta-cell may contribute to the rise in cytosol Ca(++) that leads to insulin release. To study whether interference with Na/Ca exchange is involved in this inhibition the effects of glucose were compared to those of ouabain. This substance inhibits Na/K ATPase, decreases the transmembrane Na(+) gradient in islets, and thus interferes with Na/Ca exchange. Collagenase isolated rat islets were maintained for 2 d in tissue culture with a trace amount of (45)Ca(++). Insulin release and (45)Ca(++) efflux were then measured during perifusion. In Ca(++)-deprived medium (to avoid changes in tissue specific radioactivity) 16.7 mM glucose inhibited (45)Ca(++) efflux. Initially 1 mM ouabain inhibited (45)Ca(++) efflux in a similar fashion, the onset being even faster than that of glucose. The effects of 16.7 mM glucose and ouabain were not additive, indicating that both substances may interfere with Na/Ca exchange. In the presence of Ca(++), 16.7 mM glucose induced biphasic insulin release. Ouabain alone caused a gradual increase of insulin release. Again, the effects of ouabain and 16.7 mM glucose were not additive. In contrast, at a submaximal glucose concentration (7 mM) ouabain enhanced both phases of release. An important role for Na/Ca exchange is suggested from experiments in which Ca(++) was removed at the time of glucose-stimulation (16.7 mM). The resulting marked inhibition of insulin release was completely overcome during first phase by ouabain added at the time of Ca(++) removal; second phase was restored to 60%. This could be due to the rapid inhibitory action of ouabain on Ca(++) efflux thereby preventing loss of cellular calcium critical for glucose to induce insulin release. It appears, therefore, that interference with Na/Ca exchange is an important event in the stimulation of insulin release by glucose.
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
January 1994,
Fundamental & clinical pharmacology,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
September 1996,
The American journal of physiology,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
February 1974,
Diabetologia,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
June 1995,
Endocrinology,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
January 1978,
Experientia,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
September 1998,
Diabetologia,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
December 1977,
Diabetes,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
October 1976,
FEBS letters,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
October 1980,
Research communications in chemical pathology and pharmacology,
E G Siegel, and
C B Wollheim, and
A E Renold, and
G W Sharp
October 1975,
Diabetologia,
