Biomaterial Database

Cultivation of M. leprae in cell-free, semi-synthetic media. 1980

T Murohashi

Attempts to cultivate M. leprae in cell-free, semi-synthetic media of various forms with lowered oxidation-reduction potential after due consideration of the biological significance of the acid-fastness of mycobacteria, resulted in the growth of this organism to the extent of visible to the naked eye. Studies on the nutritional requirements at 30 degrees C, suggesting to have quite similar enzyme system as other bacterial species, if not quantitatively, but at least qualitatively. Successful cultivation enabled us to provide the in vitro drug sensitivity test method by which the most adequate drugs to be administered to the concerned leprosy patients could be selected based on the results. As the growth of this organism is still slow and poor at the present step of the studies, further studies are necessary on the medium compositions to promote the multiplication of this organism.

UI	MeSH Term	Description	Entries
D009166	Mycobacterium leprae	A species of gram-positive, aerobic bacteria that causes LEPROSY in man. Its organisms are generally arranged in clumps, rounded masses, or in groups of bacilli side by side.
D002474	Cell-Free System	A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)	Cellfree System,Cell Free System,Cell-Free Systems,Cellfree Systems,System, Cell-Free,System, Cellfree,Systems, Cell-Free,Systems, Cellfree
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia