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Antibodies were raised in rabbits to electrophoretically homogeneous cytochromes P-450 isolated from rat and human liver microsomes. These antibodies were used to compare various forms of rat, rabbit, and human cytochromes P-450 present in microsomes and in purified preparations by using double-diffusion analysis, immunoelectrophoresis, quantitative microcomplement fixation, competitive radioimmune assay and inhibition of enzyme activity toward d-benzphetamine and benzo[a]pyrene. The results indicate that (1) at least some forms of cytochrome P-450 from the three species share certain common immunological determinants, (2) there are immunological differences between cytochromes P-450 isolated from the three species, (3) some immunological differences exist between cytochromes P-450 isolated from rats of different strains, (4) immunologically distinguishable forms of cytochrome P-450 exist within individual human liver samples, and (5) human liver samples obtained from different individuals contain immunologically different forms of cytochrome P-450. Quantitative microcomplement fixation techniques were used to assign immunological distances to different form of rat, rabbit, and human liver microsomal cytochrome P-450. Cross-reactivity was observed in all systems tested, and the extent of immunological similarity was dependent upon the particular assay used.
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
January 1979,
Molecular pharmacology,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
October 1988,
Xenobiotica; the fate of foreign compounds in biological systems,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
January 1984,
Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
June 1978,
Biochemical and biophysical research communications,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
July 1978,
The Journal of biological chemistry,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
January 1984,
Xenobiotica; the fate of foreign compounds in biological systems,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
December 1984,
Archives of biochemistry and biophysics,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
February 1988,
Xenobiotica; the fate of foreign compounds in biological systems,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
December 1987,
Journal of steroid biochemistry,
F P Guengerich, and
P Wang, and
P S Mason, and
M B Mitchell
August 1982,
Biochemical and biophysical research communications,
