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A modification of the mouse fetal liver cell bioassay for erythropoietin (ESF) that allows quantitative detection of ESF in human serum is described. The modification consists of (1) correction for the effect of serum iron on 59Fe incorporation into heme and (2) the use of an "internal standard," i.e., a standard ESF preparation dissolved in the assayed test serum. As a result of this modification the statistical method of bioassay analysis had to be changed fundamentally. The mean serum concentration of ESF measured in 20 normal males was 48 microunit/ml, as compared to 29 microunit/ml in 18 females. The difference was significant at p = 0.017. The stimulatory activity of a human serum on 59Fe incorporation into heme could be neutralized by an anti-human ESF immune serum.
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
November 1981,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
May 1976,
Biomedicine / [publiee pour l'A.A.I.C.I.G.],
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
January 1975,
Experimental hematology,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
June 1980,
Blood,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
April 1982,
American journal of hematology,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
January 1973,
British journal of haematology,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
May 1976,
Experimental hematology,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
February 1978,
Experimental hematology,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
November 1981,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
G de Klerk, and
A A Hart, and
C Kruiswijk, and
R Goudsmit
January 1986,
Blood cells,
