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Sepharose 4B-immobilized desialylated ovine submaxillary mucin was used as an acceptor for galactose transfer from UDP-galactose, catalyzed by a Triton X-100-solubilized galactosyltransferase from human erythrocyte ghosts. The product could be cleaved from the insoluble acceptor substrate by alkaline borohydride treatment and identified on Bio-Gel P-2 as a disaccharide. The nature of the glycosidic bond of the isolated material was elucidated by periodate oxidation/NaB[3H]4 reduction/acid hydrolysis and subsequent identification of the aminopolyol formed as L-threosaminitol. Specific cleavage of the enzymatic product by beta-galactosidase indicated a beta-configuration for incorporated galactose. These data permit classification of the enzyme as UDP-galactose: alpha-D-N-acetylgalactosaminyl-protein beta (1 leads to 3) transferase. Furthermore, in the presence of Triton X-100, the enzyme from normal erythrocytes catalyzed transfer of galactose to the glycan moieties of asialo-agalacto-glycophorin in Tn-erythrocytes from a patient with permanent mixed-field polyagglutinability.
F J Hesford, and
E G Berger, and
D H Van den Eijnden
October 1978,
European journal of biochemistry,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
August 1972,
The Journal of clinical investigation,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
October 1994,
Biochemical and biophysical research communications,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
November 1998,
FEBS letters,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
December 1988,
Biochemical and biophysical research communications,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
October 2015,
Drug testing and analysis,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
July 1987,
Indian journal of pathology & microbiology,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
April 2019,
Transfusion,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
October 1980,
Proceedings of the National Academy of Sciences of the United States of America,
F J Hesford, and
E G Berger, and
D H Van den Eijnden
June 1980,
Biochemical and biophysical research communications,
