BIOMAT Database Logo BIOMAT Database Logo

On the accuracy of protein synthesis in Drosophila melanogaster. 1981

J Parker, and J Flanagan, and J Murphy, and J Gallant

We have investigated the accuracy of protein synthesis in somatic tissue of young and senescent Drosophila melanogaster by means of two-dimensional electrophoresis. Electrophoretic heterogeneity characteristic of translational error could not be detected at either age, placing the error frequency below about 4 X 10(-4) per codon. As a positive control on the efficacy of the technique, we showed that electrophoretic heterogeneity is detectable in bacterial cells grown in streptomycin, and that the error frequency calculated from these data is in good agreement with independent measurements. Such cells can tolerate an error frequency of about 3 x 10(-3) without cell lethality, or error catastrophe. Since the Drosophila somatic error frequency is at least eight times lower, even in senescent organisms, translational error is unlikely to be a cause of senescence in Drosophila.

UI MeSH Term Description Entries
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D008297 Male Males
D004331 Drosophila melanogaster A species of fruit fly frequently used in genetics because of the large size of its chromosomes. D. melanogaster,Drosophila melanogasters,melanogaster, Drosophila
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000375 Aging The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time. Senescence,Aging, Biological,Biological Aging
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013307 Streptomycin An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis. Estreptomicina CEPA,Estreptomicina Clariana,Estreptomicina Normon,Strepto-Fatol,Strepto-Hefa,Streptomycin Grünenthal,Streptomycin Sulfate,Streptomycin Sulfate (2:3) Salt,Streptomycin Sulphate,Streptomycine Panpharma,Strepto Fatol,Strepto Hefa
D014176 Protein Biosynthesis The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS. Genetic Translation,Peptide Biosynthesis, Ribosomal,Protein Translation,Translation, Genetic,Protein Biosynthesis, Ribosomal,Protein Synthesis, Ribosomal,Ribosomal Peptide Biosynthesis,mRNA Translation,Biosynthesis, Protein,Biosynthesis, Ribosomal Peptide,Biosynthesis, Ribosomal Protein,Genetic Translations,Ribosomal Protein Biosynthesis,Ribosomal Protein Synthesis,Synthesis, Ribosomal Protein,Translation, Protein,Translation, mRNA,mRNA Translations

Related Publications

J Parker, and J Flanagan, and J Murphy, and J Gallant
Protein synthesis during spermatogenesis in Drosophila melanogaster.
January 1968, Mutation research,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Studies on protein synthesis in imaginal discs of Drosophila melanogaster.
August 1967, Experimental cell research,
J Parker, and J Flanagan, and J Murphy, and J Gallant
The effect of age on the initiation of protein synthesis in Drosophila melanogaster.
May 1981, Mechanisms of ageing and development,
J Parker, and J Flanagan, and J Murphy, and J Gallant
PROTEIN SYNTHESIS IN CELL-FREE PREPARATIONS FROM DROSOPHILA MELANOGASTER.
May 1965, The Journal of biological chemistry,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Patterns of protein synthesis in imaginal discs of Drosophila melanogaster.
December 1977, Cell,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Synthesis and deposition of yolk protein in adult Drosophila melanogaster.
January 1976, Journal of insect physiology,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Cell-free protein synthesis in lysates of Drosophila melanogaster cells.
April 1979, Biochemistry,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Decreased protein synthesis by microsomes from aging Drosophila melanogaster.
January 1979, Experimental gerontology,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Stage-specific protein synthesis during early embryogenesis in Drosophila melanogaster.
January 1983, The EMBO journal,
J Parker, and J Flanagan, and J Murphy, and J Gallant
Accuracy of bristle placement on a leg segment in Drosophila melanogaster.
November 1983, Journal of morphology,
Copied contents to your clipboard!