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An immunoenzyme assay for detection of platelet antibodies and suitable for routine use is described. Purified rabbit IgG anti-human IgG antibodies are conjugated to beta-galactosidase with meta-maleimidobenzoyl-hydroxysuccinimide ester as a bifunctional reagent and o-nitrophenyl-beta-galactopyranoside as a substrate to evaluate the enzymatic activity of the labeled antiglobulin. The sera of 26 patients suffering from various diseases (acute leukemia, aplastic anemia and systemic lupus erythematosus) and 40 control subjects were assayed with the enzyme-labeled reagent and, for comparison, with an indirect immunofluorescence technique. Half of these patients had never been transfused. Platelet antibodies were detected by both assays in all the transfused patients except one, and in 3 out of 13 non-transfused patients. The sera of all the control subjects were negative. Quantitation of platelet antibodies was obtained by a sensitive antiglobulin absorption technique. A method for standardization of the reagents allowing comparison of results obtained in the same patient at different times and suitable for long-term follow-up studies is also described.
P Borzini, and
F Tedesco, and
N Greppi, and
P Rebulla, and
A Parravicini, and
G Sirchia
January 1989,
Haematologica,
P Borzini, and
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A Parravicini, and
G Sirchia
September 1980,
Revue francaise de transfusion et immuno-hematologie,
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G Sirchia
January 1977,
Annales de microbiologie,
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G Sirchia
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Analytical biochemistry,
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A Parravicini, and
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January 2000,
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P Borzini, and
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P Rebulla, and
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G Sirchia
August 1999,
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
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G Sirchia
May 2000,
Acta tropica,
P Borzini, and
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G Sirchia
January 1994,
Archivum immunologiae et therapiae experimentalis,
P Borzini, and
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P Rebulla, and
A Parravicini, and
G Sirchia
August 1981,
American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction,
P Borzini, and
F Tedesco, and
N Greppi, and
P Rebulla, and
A Parravicini, and
G Sirchia
June 1982,
Klinische Wochenschrift,
