In a glove-box made of stainless steel germ-carriers of lime-wood and aluminium were layed out after having been contaminated with different vegetative bacteria (Staphylococcus aureus, Streptococcus faecium, Pseudomonas aeruginosa, Proteus mirabilis). Disinfectant aerosols of several germicidal substances belonging to different chemical groups were brought into the glove-box. The aerosol was produced by a commercial generator. The following substances proved to have a good disinfectant effect under the conditions used: hydrogen peroxide, peracetic acid, formaldehyde, glutardialdehyde, beta-propiolacton, Lysoformin resp. Lysoformin 2000 and Tegodor 73, whereas triethylenglycol, phenol-derivatives (Gevisol, Environ), Chloramine 80 and detergents were not able to kill all the bacteria on the germ-carriers within 2 h. Determination of the natural mortality rate showed, that at relative humidities of over 58% the test bacteria die within 72 h on the germ-carriers without presence of disinfectants. At a relative humidity of 77%, Staphylococcus aureus on wooden germ-carriers died within 24 h.