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Rat Factor II (prothrombin), isolated and purified by chromatography on Blue Dextran-agarose, was used to raise an antiserum in rabbits. On the basis of single radial immunodiffusion measurements. Factor II synthesis by isolated perfused rat liver amounted to 0.54 mg/300 cm2 body surface area of the liver donor in 10 h. Corresponding measurements of Factor II coagulant activity revealed cumulative synthesis of 802 Iowa units. Coumadin added to the liver perfusate blocked production of Factor II coagulant activity, but did not change synthesis of the immunologically measured protein. In perfusions in which either heparin or citrate was used as anticoagulant, synthesis of albumin was not affected by the choice of anticoagulant but bile production and synthesis of Factor II were significantly less in citrate perfusions.
M R Owens, and
L L Miller, and
C D Cimino
April 1979,
British journal of haematology,
M R Owens, and
L L Miller, and
C D Cimino
September 1983,
The Journal of clinical investigation,
M R Owens, and
L L Miller, and
C D Cimino
June 1982,
Blood,
M R Owens, and
L L Miller, and
C D Cimino
January 1975,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M R Owens, and
L L Miller, and
C D Cimino
May 1966,
The Journal of clinical investigation,
M R Owens, and
L L Miller, and
C D Cimino
August 1964,
Biochimica et biophysica acta,
M R Owens, and
L L Miller, and
C D Cimino
December 1966,
Biochimica et biophysica acta,
M R Owens, and
L L Miller, and
C D Cimino
April 1966,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
M R Owens, and
L L Miller, and
C D Cimino
November 1960,
Biochemical and biophysical research communications,
M R Owens, and
L L Miller, and
C D Cimino
July 1970,
Nature,
