-transparent.png){kind=logo}
A radiometric method is described for measuring pyrimidine 5'-nucleotidase activity in human peripheral blood haemolysate. The substrate [14C]uridine monophosphate is converted to [14C]uridine. Two simple and rapid methods to isolate the product of the reaction employing either descending chromatography or elution from DEAE cellulose paper have been developed. With the described methods the specific activity of pyrimidine 5'-nucleotidase of human erythrocytes is 6.7 +/- 2.7 (+/- 1 S.D.) nmol/h/mg protein and this activity appears to be widely distributed in human tissue.
Related Publications
P H Ellims, and
L Bailey, and
M B Van der Weyden
August 1980,
British journal of haematology,
P H Ellims, and
L Bailey, and
M B Van der Weyden
December 1985,
The Journal of laboratory and clinical medicine,
P H Ellims, and
L Bailey, and
M B Van der Weyden
January 2002,
Archives of biochemistry and biophysics,
P H Ellims, and
L Bailey, and
M B Van der Weyden
January 1981,
Enzyme,
P H Ellims, and
L Bailey, and
M B Van der Weyden
January 1987,
British journal of haematology,
P H Ellims, and
L Bailey, and
M B Van der Weyden
January 1986,
Industrial health,
P H Ellims, and
L Bailey, and
M B Van der Weyden
November 1982,
Analytical biochemistry,
P H Ellims, and
L Bailey, and
M B Van der Weyden
September 1977,
Proceedings of the National Academy of Sciences of the United States of America,
P H Ellims, and
L Bailey, and
M B Van der Weyden
January 1978,
Monographs in human genetics,
P H Ellims, and
L Bailey, and
M B Van der Weyden
July 2005,
Cellular and molecular life sciences : CMLS,
