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We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to inactivate the enzyme. The antibody-bound fraction is then precipitated with polyethylene glycol and isolated by centrifugation. This easy method eliminates extraction and purification and gives accurate and reliable results. The total time required for 100 estimations, including counting time, is about 6 h.
T P Lee, and
C H Tan
February 1983,
Clinical chemistry,
T P Lee, and
C H Tan
February 1996,
Journal of immunoassay,
T P Lee, and
C H Tan
January 1975,
The Journal of nuclear biology and medicine,
T P Lee, and
C H Tan
May 1981,
Journal of steroid biochemistry,
T P Lee, and
C H Tan
March 1974,
Clinical chemistry,
T P Lee, and
C H Tan
November 1983,
Clinical chemistry,
T P Lee, and
C H Tan
June 1972,
The Journal of clinical endocrinology and metabolism,
T P Lee, and
C H Tan
December 1976,
Scandinavian journal of clinical and laboratory investigation,
T P Lee, and
C H Tan
October 1980,
Clinical chemistry,
T P Lee, and
C H Tan
February 1974,
The Indian journal of medical research,
