Biomaterial Database

Distribution of DNA-bound carcinogen 4-nitroquinoline 1-oxide and of repair-synthesized DNA in chromatin of WI-38 cells. 1981

K Nose

Distribution of DNA-bound 4-nitroquinoline 1-oxide (4NQO) and repair-synthesized DNA after treatment with 4NQO in chromatin was investigated with human diploid fibroblast WI-38. Cells were incubated with [3H] 4NQO, and sites of binding on chromatin DNA were analyzed either by sensitivity to micrococcal nuclease or DNase I, or by fractionation of chromatin on sucrose gradient centrifugation. The results showed that 4NQO preferentially binds to DNA of linker region of chromatin, but the binding was random with respect to transcriptional activity of chromatin. The distribution of repair-synthesized DNA in chromatin damaged by 4NQO was also studied by similar experiments. [3H] Thymidine incorporated into repaired DNA was more sensitive to nucleases, but distributed almost equally among DNAs of chromatin subfractions with different transcriptional activity.

UI	MeSH Term	Description	Entries
D009600	Nitroquinolines	Quinolines substituted in any position by one or more nitro groups.
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002843	Chromatin	The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.	Chromatins
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004260	DNA Repair	The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones.	DNA Damage Response
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D015112	4-Nitroquinoline-1-oxide	A potent mutagen and carcinogen. This compound and its metabolite 4-HYDROXYAMINOQUINOLINE-1-OXIDE bind to nucleic acids. It inactivates bacteria but not bacteriophage.	4-Nitroquinoline-N-oxide,4 Nitroquinoline 1 oxide,4 Nitroquinoline N oxide