[Conditions for nisin adsorption by Streptococcus lactis cells]. 1981

V A Grushina, and I P Baranova, and N S Egorov

Some conditions for absorption of nisin, a polypeptide antibiotic by the cells of Str. lactis were studied. The amounts of nisin adsorbed by the cells depended on the culture age: at the late stationary phase the adsorption level was 2 times higher than that at the logarithmic phase. The cells grown on a "poor" medium adsorbed 85-90 per cent of nisin added to the solution, while the cells grown on the "rich" medium adsorbed 50 per cent of the antibiotic. The adsorption level of nisin by the cells subjected to a thermal shock was higher than that by the live cells. Desorption of nisin from the cells with acid ethanol and bivalent cation solutions was insignificant. Nisin is adsorbed by the cells of other microorganisms, the adsorption levels by the cells of Bac. brevis being the same as those by the streptococcal cells, while the levels adsorbed by Bac. polymyxa being 4 times lower.

UI MeSH Term Description Entries
D009561 Nisin A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000327 Adsorption The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily. Adsorptions
D001407 Bacillus A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic. Bacillus bacterium
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution
D013294 Lactococcus lactis A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID. Streptococcus lactis,Lactococcus lactis subsp. lactis
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

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