Biomaterial Database

Regression line analysis of neutrophil chemotaxis. 1982

T E Van Dyke, and A A Reilly, and R J Genco

Polymorphonuclear leukocyte chemotaxis and chemokinesis, in the micropore filter assay, have been evaluated using Regression Line Analysis. The method discriminates between migration rate and the number of migrating cells by statistical comparison of the slope of the regression line and the y-intercept, respectively. The derivation of this new method of assessment of cell migration is reported. This report also includes critical evaluation of the chemokinesis dose/response curve and the directional component of chemotaxis. The effect of inhibitors of chemotaxis were evaluated using the new method. Colchicine was found to effect the directional component of chemotaxis, as indicated by decreased rate of locomotion in chemotaxis experiments, and had no effect on random migration and chemokinesis. Cytochalasin B had a general inhibitory effect upon cell locomotion by depressing the rate of random migration and chemotaxis. 1-Tosylamide-2-phenylethyl chloromethylketone (TPCK) had the apparent effect of uncoupling the receptor and effector mechanisms of chemotaxis or altering the binding of the chemotactic ligand, since random migration was unaffected but the rate of chemotaxis and chemokinesis were depressed. Ionophore A23187 had a generalized inhibitory effect on the cell rate under all three conditions; however, at low concentrations it induced an increase in the number of migrating cells. These studies indicate that the method of regression line analysis is effective in differentiating in vitro the mechanisms of chemotaxis inhibition. The method may be used effectively in the assessment of defective neutrophil chemotaxis in disease states and may prove a useful adjunct to the study of cell locomotory mechanisms.

UI	MeSH Term	Description	Entries
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D012044	Regression Analysis	Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable.	Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D002634	Chemotaxis, Leukocyte	The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.	Leukotaxis,Leukocyte Chemotaxis
D003078	Colchicine	A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).	Colchicine, (+-)-Isomer,Colchicine, (R)-Isomer
D003571	Cytochalasin B	A cytotoxic member of the CYTOCHALASINS.	Phomin
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000001	Calcimycin	An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.	4-Benzoxazolecarboxylic acid, 5-(methylamino)-2-((3,9,11-trimethyl-8-(1-methyl-2-oxo-2-(1H-pyrrol-2-yl)ethyl)-1,7-dioxaspiro(5.5)undec-2-yl)methyl)-, (6S-(6alpha(2S,3S),8beta(R*),9beta,11alpha))-,A-23187,A23187,Antibiotic A23187,A 23187,A23187, Antibiotic
D014108	Tosylphenylalanyl Chloromethyl Ketone	An inhibitor of Serine Endopeptidases. Acts as alkylating agent and is known to interfere with the translation process.	Chlorophenyltosylamidobutanone,TPCK,Tosylphenylalanyl Chloromethane,Chloromethane, Tosylphenylalanyl,Chloromethyl Ketone, Tosylphenylalanyl,Ketone, Tosylphenylalanyl Chloromethyl
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In