Biomaterial Database

The in vitro metabolism of mevalonate by sterol and nonsterol pathways in neonatal chick. 1982

J A Aguilera, and A Linares, and V Arce, and E García-Peregrín

1. Incorporation of mevalonate into nonsaponifiable lipids by neonatal chick liver and kidney slices was studied as a function of weight of tissue and incubation time. In the same conditions, more nonsaponifiable lipids were produced in kidney than in liver. 2. CO2 production from mevalonate increased with the weight of tissue and with the incubation time. Over 80% of CO2 produced from mevalonate resulted from the shunt pathway in kidney, while in liver this route was quantitatively insignificant. 3. 2-14C from mevalonate was also incorporated into saponifiable (acidic) fraction by kidney slices. 4. Incorporation into nonsaponifiable lipids and total CO2 increased with the concentration of mevalonate. This increase was more pronounced in kidney than in liver especially at low mevalonate concentrations. In the presence of 0.1-8.0 mM mevalonate over 80% of the CO2 produced from this substrate resulted from the shunt pathway, while liver was practically ineffective whatever be the concentration of mevalonate. 5. The percentage of mevalonate metabolized in the kidney by shunt pathway increased with the incubation time and reached over 15% in the presence of 0.1-8.0 mM mevalonate.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D008055	Lipids	A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)	Lipid
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D008798	Mevalonic Acid	A dihydroxy monocarboxylic acid and precursor in the biosynthetic pathway known as the mevalonate pathway, which produces terpenes and steroids that are vital for diverse cellular functions.	Mevalonate,Acid, Mevalonic
D009928	Organ Specificity	Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.	Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D002245	Carbon Dioxide	A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.	Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia