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The method for long-term culture of marrow cells in vitro as described by Dexter has recently been successfully applied to human marrow and is dependent on the development of an adherent stromal cell layer consisting of cells described as "endothelial-like cells, fat cells, and macrophages". The present study was designed to determine the origin and composition of the stromal cells forming the in vitro 'microenvironment' and maintaining haematopoiesis in long-term cultures grown from marrows of 14 patients who received marrow transplants from HLA identical siblings of the opposite sex. The presence of a Y chromosome was used as a marker to establish the donor or recipient origin of the cells. We found that the stromal cells became progressively donor in origin with time after transplantation and some reacted with antibody directed against factor VIII-associated antigen. In addition, donor-derived in vitro stromal cells synthesized both interstitial and basal lamina collagen types, indicating that the in vitro microenvironment is transplantable and composed in part of endothelial-like cells.
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
April 1984,
Experientia,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
November 1984,
International journal of cell cloning,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
January 2000,
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
December 1983,
The New England journal of medicine,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
December 2009,
Clinical and experimental dermatology,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
January 1993,
Haematologica,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
April 2006,
Zhongguo shi yan xue ye xue za zhi,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
June 2013,
The European respiratory journal,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
November 1999,
Transplantation,
A Keating, and
J W Singer, and
P D Killen, and
G E Striker, and
A C Salo, and
J Sanders, and
E D Thomas, and
D Thorning, and
P J Fialkow
October 2003,
American journal of clinical pathology,
