Biomaterial Database

A simple and reproducible method for the estimation of triiodothyronine uptake (thyroxine binding index) using a new adsorbent. 1982

J D Mantzos, and P P Yialouris

A simple, rapid and reproducible method for the determination of triiodothyronine uptake using heat-treated wheat flour (TWF) as adsorbent is described. Three ml of TWF suspension in barbital buffer containing 125I triiodothyronine is added to 0.1 ml of serum sample in a plastic tube. The mixture is shaken for 30 seconds and is left to settle for 12 minutes or alternatively is centrifuged for one minute at 600 X g. The 1.0 ml of supernatant is transferred to another tube for counting. This method is virtually time and temperature independent. The effects of altering the concentration of 125I triiodothyronine and TWF are reported. Within-run precision has been estimated for normal (C.V. 1.2), high (C.V. 1.2) and low (C.V. 1.8) ranges. Estimation of between -run precision gave similar results. Comparison of results obtained by this method and by Thyopac-3 reagent kit (Radiochemical Centre, Amersham, England) shows good correlation for Thyopac-3 values about 105, while for values below 105 the present method gives lower values than Thyopac-3.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D005433	Flour	A powdery meal obtained from ground up starchy foodstuff, such as seeds of WHEAT; RICE and other EDIBLE GRAIN; LEGUMES; and NUTS; or ground up PLANT TUBERS.	Almond Flour,Almond Meal,Corn Flour,Corn Meal,Grain Flour,Grain Meal,Nut Flour,Potato Flour,Rice Flour,Rice Meal,Soybean Flour,Soybean Meal,Wheat Flour,Wheat Meal,Semolina Flour,Almond Flours,Almond Meals,Corn Flours,Corn Meals,Flour, Almond,Flour, Corn,Flour, Grain,Flour, Nut,Flour, Potato,Flour, Rice,Flour, Semolina,Flour, Soybean,Flour, Wheat,Flours,Flours, Almond,Flours, Corn,Flours, Grain,Flours, Nut,Flours, Potato,Flours, Rice,Flours, Semolina,Flours, Soybean,Flours, Wheat,Grain Flours,Grain Meals,Meal, Almond,Meal, Corn,Meal, Grain,Meal, Rice,Meal, Soybean,Meal, Wheat,Meals, Almond,Meals, Corn,Meals, Grain,Meals, Rice,Meals, Soybean,Meals, Wheat,Nut Flours,Potato Flours,Rice Flours,Rice Meals,Semolina Flours,Soybean Flours,Soybean Meals,Wheat Flours,Wheat Meals
D000327	Adsorption	The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.	Adsorptions
D013975	Thyroxine-Binding Proteins	Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.	Thyroxine Transport Protein,Thyroxine-Binding Protein,Thyroxine Binding Protein,Thyroxine Binding Proteins
D014284	Triiodothyronine	A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.	Liothyronine,T3 Thyroid Hormone,3,3',5-Triiodothyronine,Cytomel,Liothyronine Sodium,Thyroid Hormone, T3
D014908	Triticum	A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.	Wheat,Durum Wheat,Triticum aestivum,Triticum durum,Triticum spelta,Triticum turgidum,Triticum turgidum subsp. durum,Triticum vulgare,Durum Wheats,Wheat, Durum