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The influence of divalent cations on glycosphingolipid metabolism was examined in the NB41A mouse neuroblastoma clonal cell line. HPLC methods were utilized to quantitate the effects on neutral glycolipids and monosialogangliosides. NB41A cells were shown to contain GM3, GM2, GM1, GD3, and GD1a by HPLC and TLC. The neutral glycosphingolipids consisted of glucosylceramide (GlcCer), lactosylceramide (LacCer), GalNAc (beta 1 leads to 4) Gal(beta 1 leads to 4)Glc(beta 1 leads to 1)Cer (GgOse3Cer), and GalNAc(beta 1 leads to 3)Gal(alpha 1 leads to 4) Gal(beta 1 leads to 4)Glc(beta 1 leads to 1)Cer (GbOse4Cer) according to their HPLC behavior. Cells grown in the presence of 1.85 mM-EGTA showed a two-to threefold increase in GM3 whereas other glycosphingolipids were only slightly affected. When cells were grown in the presence of 1.45 mM-EGTA plus 0.4 mM-EDTA a similar increase in GM3 was observed but this change was now accompanied by decreases in GM2, GM1, GgOse3Cer. The EGTA-EDTA effects were reversed when growth was in the presence of Ca2+ sufficient to bind all chelator. Mn2+ replacement reversed the chelator effects differentially; GM2 and GM1 levels were the most sensitive to increases in Mn2+ concentration; GgOse3Cer and GbOse4Cer were also sensitive, whereas GM3 was the least affected. These results suggest calcium serves an important regulatory role on GM3 levels and that manganese concentration may regulate the levels of galactosamine-containing glycolipids in mouse NB41A neuroblastoma cells.
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
September 1995,
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E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
August 1995,
Toxicology in vitro : an international journal published in association with BIBRA,
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
August 1974,
Developmental biology,
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
September 1953,
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E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
January 1985,
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E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
January 1970,
Proceedings of the National Academy of Sciences of the United States of America,
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
October 2001,
Fundamental & clinical pharmacology,
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
November 1974,
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E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
July 1988,
European journal of pharmacology,
E G Bremer, and
V S Sapirstein, and
T Savage, and
R H McCluer
May 1974,
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