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Membrane-bound mRNA was isolated from Raji cells and enriched for message coding for the HLA-DR transplantation antigen alpha-chain by sucrose gradient centrifugation. Double-stranded cDNA was constructed from this mRNA fraction, ligated to plasmid pBR322, and cloned into Escherichia coli. By hybrid selection, a plasmid, pDR-alpha-1, able to hybridize with mRNA coding for the HLA-DR alpha-chain was identified. From the nucleotide sequence of one end of the insert an amino acid sequence was predicted which is identical to part of the amino-terminal sequence of an HLA-DR alpha-chain preparation isolated from Raji cells. This clearly shows that pDR-alpha-1 carries almost the complete message for an HLD-DR alpha-chain. From the nucleotide sequence of this plasmid it will be possible to predict the primary structure of an HLA-DR alpha-chain.
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
October 1982,
Proceedings of the National Academy of Sciences of the United States of America,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
January 1984,
Nature,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
October 1982,
Nature,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
January 1983,
The EMBO journal,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
June 1984,
Proceedings of the National Academy of Sciences of the United States of America,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
February 1984,
Molecular biology & medicine,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
September 1985,
Journal of immunology (Baltimore, Md. : 1950),
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
K Gustafsson, and
P Bill, and
D Larhammar, and
K Wiman, and
L Claesson, and
L Schenning, and
B Servenius, and
J Sundelin, and
L Rask, and
P A Peterson
November 1982,
Proceedings of the National Academy of Sciences of the United States of America,
