Biomaterial Database

Comparative evaluation of various methods of detecting antibodies against soluble nuclear antigens. 1982

I Zimmermann-Górska, and U Potocka-Michajluk

Results of anti-ENA antibody employing double diffusion in agar gel, electroimmunoprecipitation (CIE) or passive hemagglutination were compared. The studies were performed on 164 patients including 79 patients with rheumatoid arthritis (RA), 46 patients with systemic lupus erythematosus (SLE), 25 patients with systemic sclerosis (PSS), 13 patients with polymyositis (PM) 5 patients with MCTD and on 30 healthy individuals. The obtained data prompt us to recommend demonstration and identification of anti-ENA antibodies in a screening type CIE assay followed by determination of their titer by hemagglutination test.

UI	MeSH Term	Description	Entries
D007122	Immunoelectrophoresis	A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D008297	Male		Males
D009698	Nucleoproteins	Proteins conjugated with nucleic acids.	Nucleoprotein
D003240	Connective Tissue Diseases	A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.	Connective Tissue Disease,Disease, Connective Tissue,Diseases, Connective Tissue
D005260	Female		Females
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006386	Hemagglutination Tests	Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)	Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody