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Determination of proteolytic activities gains increasing importance for the diagnosis of various pathologic conditions. Many questions can only be solved with the aid of experiments with animals. In preparation of such studies the pattern of the enzymatic hydrolysis of 20 amino acid-p-nitroanilides and one -beta-naphthylamide by normal rat serum was measured. The pH dependences of the single reactions are reported as well as their sensitivity to the action of ethylenediaminetetraacetic acid, dithioerithrol, and puromycin. Additionally, it is shown that collecting of the blood by cardiac puncture and preparation of the native plasma is the best way to get reproducible results.
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
July 1977,
Clinica chimica acta; international journal of clinical chemistry,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
April 1980,
Rinsho byori. The Japanese journal of clinical pathology,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
March 1971,
Journal of bacteriology,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
April 1972,
Veterinarni medicina,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
March 1974,
Clinica chimica acta; international journal of clinical chemistry,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
November 1962,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
November 1971,
Journal of bacteriology,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
January 1982,
Sangyo igaku. Japanese journal of industrial health,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
October 1990,
The Journal of biological chemistry,
R Birnbaum, and
T Unger, and
M Nagelschmidt, and
H Struck
June 1971,
Journal of biochemistry,
