-transparent.png){kind=logo}
In order to further investigate the molecular basis for the action of TRH, experiments were performed to examine the ability of rat anterior pituitary cells to incorporate [32P]orthophosphate into phospholipids in response to the neurohormone. Addition of 0.1 microM TRH rapidly stimulates the phosphatidylinositol (PI) response. The incorporation of radioactivity into PA and PI is increased as early as in 2 and 5 min after TRH addition, respectively, and reaches 40 and 140% above control, respectively, at 20 min. By contrast, 0.1 microM somatostatin is inactive. TRH exerts its stimulatory effect at an ED50 value of ca. 10 nM. The present results suggest that the PI response is an early event associated with the action of TRH in the anterior pituitary gland.
P C Leung, and
V Raymond, and
F Labrie
January 1985,
The American journal of physiology,
P C Leung, and
V Raymond, and
F Labrie
June 1994,
Biochemical and biophysical research communications,
P C Leung, and
V Raymond, and
F Labrie
January 1974,
Problemes actuels d'endocrinologie et de nutrition,
P C Leung, and
V Raymond, and
F Labrie
April 1982,
Endocrinology,
P C Leung, and
V Raymond, and
F Labrie
June 1994,
Biochemical and biophysical research communications,
P C Leung, and
V Raymond, and
F Labrie
April 2004,
Acta physiologica Scandinavica,
P C Leung, and
V Raymond, and
F Labrie
January 1987,
Neuroendocrinology,
P C Leung, and
V Raymond, and
F Labrie
October 1981,
Physiological reviews,
P C Leung, and
V Raymond, and
F Labrie
June 2005,
Acta physiologica Scandinavica,
P C Leung, and
V Raymond, and
F Labrie
January 1974,
Prostaglandins,
