Primary structure of a novel N-glycosidic carbohydrate unit, derived from hen ovomucoid. A 500-MHz 1H-NMR study. 1983

J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart

The N-glycosidic carbohydrate chains of hen beta-ovomucoid were released from the protein by hydrazinolysis, and separated by HPLC. Primary structural analysis of 3 major fractions was conducted by applying 500-MHz 1H-NMR spectroscopy in combination with methylation analysis. One of the fractions investigated appeared to consist of an intersected penta-antennary structure extended with one Gal residue. The location of the latter in a certain branch could be established unambiguously by NMR. This structure is a novel member of the family of N-glycosidic carbohydrates of glycoproteins.

UI MeSH Term Description Entries
D008745 Methylation Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed) Methylations
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D010059 Ovomucin A heterogeneous mixture of glycoproteins responsible for the gel structure of egg white. It has trypsin-inhibiting activity. Ovomucoid
D002621 Chemistry A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D002627 Chemistry, Physical The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes. Physical Chemistry,Chemistries, Physical,Physical Chemistries
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004527 Egg Proteins Proteins which are found in eggs (OVA) from any species. Egg Protein,Egg Shell Protein,Egg Shell Proteins,Egg White Protein,Egg White Proteins,Egg Yolk Protein,Egg Yolk Proteins,Ovum Protein,Ovum Proteins,Yolk Protein,Yolk Proteins,Protein, Egg,Protein, Egg Shell,Protein, Egg White,Protein, Egg Yolk,Protein, Ovum,Protein, Yolk,Proteins, Egg,Proteins, Egg Shell,Proteins, Egg White,Proteins, Egg Yolk,Proteins, Ovum,Proteins, Yolk,Shell Protein, Egg,Shell Proteins, Egg,White Protein, Egg,White Proteins, Egg,Yolk Protein, Egg,Yolk Proteins, Egg
D005260 Female Females
D006027 Glycosides Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed) Glycoside

Related Publications

J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
July 1987, European journal of biochemistry,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
October 1981, FEBS letters,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
September 1988, Biopolymers,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
November 1980, FEBS letters,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
November 1980, FEBS letters,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
May 1988, Carbohydrate research,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
September 1982, European journal of biochemistry,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
April 1989, Analytical biochemistry,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
May 1985, Biochemistry,
J Paz-Parente, and G Strecker, and Y Leroy, and J Montreuil, and B Fournet, and H van Halbeek, and L Dorland, and J F Vliegenthart
May 1985, Nucleic acids research,
Copied contents to your clipboard!