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The specificity of five monoclonal antibodies (P1-P6) against platelet surface components was determined by immunoprecipitation of surface-labelled platelets from normal donors and patients with known platelet glycoprotein defects, followed by analysis by gel electrophoresis. Three (P2, P4 and P6) precipitated glycoproteins IIb and IIIa and, in addition, P2 precipitated glycoprotein Ia. P1 precipitated normally only glycoprotein Ib also Ia when the platelets were pretreated with neuraminidase. P3 precipitated principally glycoprotein Ia but glycoprotein Ib was also weakly precipitated. The effects of the monoclonals on platelet function were tested. P1 and P2 completely inhibited and P3 slightly inhibited thrombin-induced platelet aggregation. P2 also inhibited collagen-induced aggregation and partially inhibited ADP-induced platelet aggregation. P3, P4 and P6 partially inhibited ADP-induced platelet aggregation. None had any effect on ristocetin-induced aggregation despite P1 and P3 binding to glycoprotein Ib. These results confirm the role of glycoproteins IIb and IIIa in aggregation induced by various agents and suggest that the function of glycoprotein Ib in thrombin-induced aggregation is more important than previously suspected and that glycoprotein Ia may also be involved in platelet functions.
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
June 1985,
Thrombosis research,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
July 1993,
Transfusion medicine reviews,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
November 1988,
Blood,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
September 1990,
European journal of haematology,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
August 1987,
Blood,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
July 2000,
Thrombosis and haemostasis,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
January 1991,
Arteriosclerosis and thrombosis : a journal of vascular biology,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
March 1983,
British journal of haematology,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
January 1990,
Archivos de investigacion medica,
J L McGregor, and
J Brochier, and
F Wild, and
G Follea, and
M C Trzeciak, and
E James, and
M Dechavanne, and
L McGregor, and
K J Clemetson
May 1997,
Journal of the American Society of Nephrology : JASN,
