Biomaterial Database

Measurement of phagocytosis utilizing 51Cr-labeled tannic acid treated erythrocytes. 1983

D Biegel, and M Rabinovitch

Macrophages recognize and ingest tannic acid treated erythrocytes (TAE) by a mechanism which does not involve the well characterized receptors for immunoglobulin and complement. Since the TAE are susceptible to osmotic lysis, a clear distinction can be made between erythrocytes which are ingested from those attached to the phagocytes' surface. We present a quantitative assay for macrophage phagocytosis using 51Cr-labeled TAE. Uptake of TAE was linearly related to the incubation time during the first 40 min, and increased with the TAE concentration in a log fashion. Furthermore, both attachment and ingestion were positively related to the concentration of tannic acid used to pretreat the erythrocytes. As an example of the application of the method, we compared the ingestion of TAE with that of opsonized erythrocytes (EA) by resident and elicited mouse peritoneal macrophages. While the uptake of EA by thioglycollate-induced macrophages was increased by a factor of approximately 7 over the resident population, the uptake of TAE was only stimulated 2.3-fold. The method will be useful in comparative physiological and pharmacological studies of immunological and non-immunological phagocytosis by macrophages.

UI	MeSH Term	Description	Entries
D008264	Macrophages	The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)	Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002860	Chromium Radioisotopes	Unstable isotopes of chromium that decay or disintegrate emitting radiation. Cr atoms with atomic weights of 46-49, 51, 55, and 56 are radioactive chromium isotopes.	Radioisotopes, Chromium
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012756	Sheep	Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.	Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D047348	Hydrolyzable Tannins	Polymeric derivatives of GALLIC ACID that are esters of a sugar.	Ellagi-Tannins,Ellagitannins,Gallo-Tannins,Gallotannins,Pyrogallol Tannins,Ellagi Tannins,Gallo Tannins,Tannins, Hydrolyzable,Tannins, Pyrogallol
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus