-transparent.png){kind=logo}
In order to explain the difference of inhibition of dihydrofolate reductase (DHFR) by methotrexate (MTX) and its metabolites 7-hydroxymethotrexate [7OH (MTX)] and polyglutamate derivatives [MTX (G1) and MTX (G2)], direct determinations of binding parameters to beef liver DHFR were performed. Association constants are calculated by fluorescence titrations and thermodynamic parameters by microcalorimetric measurements. The parameters of interaction are nearly identical for MTX and polyglutamate derivatives but are different for MTX and 7OH (MTX). For this last derivative electrostatic forces are less predominant and a larger modification of its conformation appears in the enzyme during the complex formation.
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
July 1977,
Science (New York, N.Y.),
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
October 1986,
Cancer research,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
January 1983,
Biochemistry,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
January 1976,
Archives of biochemistry and biophysics,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
October 2002,
Proceedings of the National Academy of Sciences of the United States of America,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
July 1980,
Nature,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
March 1998,
Journal of molecular biology,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
November 1985,
Annals of clinical biochemistry,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
July 1978,
Proceedings of the National Academy of Sciences of the United States of America,
M Chauvet, and
M Bourdeaux, and
C Briand, and
M Dell'Amico, and
R Gilli, and
M Diarra
June 1978,
Biochemistry,
