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A semi-automatic system based on flow injection analysis (FIA) for the transportation of small sample aliquots has been combined with fluorometric, enzymatic methods for blood lactate determination and has been described earlier. In the present study duplicate blood samples were obtained from exercising subjects to enable a comparison of lactate concentrations between neutralized and non-neutralized samples. Duplicate samples were also obtained to enable FIA lactate values to be compared to those obtained with a manual enzymatic method, and with a colorimetric method. No significant change was observed if the sample was not neutralized, enabling a more rapid sample turnover. The FIA method was reliable, with a coefficient of variation of 4.9% between duplicate blood samples. FIA lactate values were valid when compared to two other manual assays. FIA has been shown to be a rapid (60 samples x h-1) means of accurately determining blood lactate concentrations with 25 microliter blood samples and is of particular relevance to the exercise laboratory.
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
December 1979,
Lakartidningen,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
January 1979,
Annali dell'Ospedale Maria Vittoria di Torino,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
February 1963,
Medecine et laboratoire,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
May 1958,
Ceskoslovenska fysiologie,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
January 1966,
Annales de biologie clinique,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
January 1957,
British journal of anaesthesia,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
January 1979,
Annali dell'Ospedale Maria Vittoria di Torino,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
January 1934,
American journal of public health and the nation's health,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
December 1978,
Revue francaise de transfusion et immuno-hematologie,
J Karlsson, and
I Jacobs, and
B Sjödin, and
P Tesch, and
P Kaiser, and
O Sahl, and
B Karlberg
April 1968,
The New England journal of medicine,
