Intravenous solutions of glucose oligosaccharides are potential sources of carbohydrate-derived energy for patients requiring intravenous feeding. Relatively little is known about utilization of glucose oligosaccharides linked by beta-glucosidic bonds. We compared the utilization of maltose (alpha-D-glucosyl-1,4-D-glucose) and beta-cellobiose (beta-D-glucosyl-1,4-D-glucose) when administered intravenously (19 g per day) to young pigs for a 5-day period. Animals infused with maltose excreted 15% of the infused disaccharide over the 5-day infusion period. No evidence of maltose accumulation was noted in plasma, and kidney morphology was normal. Animals infused with beta-cellobiose excreted 95% of the infused disaccharide in the urine. The mean (+/- SD) plasma total glucose concentration increased significantly over base-line values of 114 +/- 39 mg/dl to a value of 180 +/- 28 mg/dl during cellobiose infusion, indicating accumulation of cellobiose in body water. Kidney morphology in cellobiose-infused animals was normal. Intravenously infused beta-cellobiose is poorly utilized by the pig when compared with the utilization of its alpha-1,4 linked isomer, maltose.