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Specific antibody-secreting hybridomas have been obtained by fusing Syrian or Armenian hamster (Mesocricetus auratus or Cricetulus migratorius) spleen cells with mouse myeloma cells. The hamsters were immunized to mouse cytolytic T lymphocytes. Hybrids were selected either by an indirect binding assay using an 125I-monoclonal antibody (MAb) reactive with hamster kappa-chains or by their ability to block T cell-mediated cytolysis. Three hybridoma clones were obtained that secreted intact IgM-like and IgG-like hamster MAb as shown by SDS-PAGE. The clones were stable as shown by subcloning. Two MAb recognized antigens of wide tissue distribution; the third bound specifically to T lymphocytes, gave strong inhibition of T cell-mediated cytolysis, and immunoprecipitated the Lyt-2,3 molecule.
F Sanchez-Madrid, and
P Szklut, and
T A Springer
January 1996,
Pflugers Archiv : European journal of physiology,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
July 1990,
Journal of immunology (Baltimore, Md. : 1950),
F Sanchez-Madrid, and
P Szklut, and
T A Springer
September 1980,
Proceedings of the National Academy of Sciences of the United States of America,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
January 1986,
Methods in enzymology,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
June 1988,
Science (New York, N.Y.),
F Sanchez-Madrid, and
P Szklut, and
T A Springer
January 1985,
Voprosy virusologii,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
January 1987,
Cancer investigation,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
November 1988,
Acta virologica,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
June 1985,
Shi yan sheng wu xue bao,
F Sanchez-Madrid, and
P Szklut, and
T A Springer
December 1983,
Gan to kagaku ryoho. Cancer & chemotherapy,
