-transparent.png){kind=logo}
Exposure of human fibroblasts to 5 J/m2 of UV light resulted in a rapid increase of up to 1500% in the intracellular content of poly(ADP-ribose) and a rapid depletion of its metabolic precursor, NAD. When added just prior to UV treatment, the poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide, totally blocked both the increase of poly(ADP-ribose) and decrease in NAD for up to 2.5 h. Addition of 3-aminobenzamide at the time of maximal accumulation of poly(ADP-ribose) resulted in a decrease to basal levels with a half-life of approximately 6 min. The rates of accumulation of poly(ADP-ribose) and depletion of NAD were increased in the presence of either 1-beta-arabinofuranosylcytosine or hydroxyurea. Since these agents are known to cause an additional accumulation of DNA strand breaks following UV irradiation, these data provide evidence for a mechanism in which the rate of poly(ADP-ribose) synthesis following DNA damage is regulated in intact cells by the number of DNA strand breaks. Under conditions in which the synthesis of poly(ADP-ribose) was blocked, DNA repair replication induced by UV light was neither stimulated nor inhibited.
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
August 2020,
Acta tropica,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
August 2001,
Experimental cell research,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
April 2005,
Cellular and molecular life sciences : CMLS,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
April 2005,
Cellular and molecular life sciences : CMLS,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
January 2016,
Current protein & peptide science,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
February 2007,
European journal of clinical investigation,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
April 2007,
Biochemical and biophysical research communications,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
May 1982,
Experimental cell research,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
August 1984,
Biochemical and biophysical research communications,
E L Jacobson, and
K M Antol, and
H Juarez-Salinas, and
M K Jacobson
November 2006,
Seikagaku. The Journal of Japanese Biochemical Society,
