-transparent.png){kind=logo}
The DNA synthesis system of freshly isolated tonsillar lymphocytes and those stimulated by phytohaemagglutinin were compared by different methods. Both cell populations had high DNA polymerase alpha and thymidine kinase activities, as well as a high rate of incorporation of [3H]thymidine into DNA. However, the two cell populations differed when their DNA distributions were compared by flow cytometry. Freshly isolated cells contained many less (6%) cells in S phase than were found in phytohaemagglutinin-stimulated lymphocytes (18%) as detected by flow cytometry. The labelling of different subpopulations of lymphocytes was studied by sorting them electrically with a fluorescence-activated cell sorter. Analysis of the radioactivity of [3H]thymidine pulse-labelled cells, sorted according to their DNA content, showed that cells in the G1 peak of DNA distribution had a significant amount of incorporated [3H]thymidine. Sorting of cells according to their size (i.e., by light scattering) revealed that only large cells were labelled with [3H]thymidine.
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
February 1978,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
July 2007,
Cytometry. Part B, Clinical cytometry,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
August 1986,
Nihon Jibiinkoka Gakkai kaiho,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
May 1971,
The Journal of laboratory and clinical medicine,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
January 1980,
Acta medica Polona,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
November 1982,
Veterinary immunology and immunopathology,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
October 1983,
Antimicrobial agents and chemotherapy,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
January 1972,
Acta biologica Academiae Scientiarum Hungaricae,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
January 1980,
Cell and tissue kinetics,
M Sasvári-Székely, and
G Szabó, and
M Staub, and
T Spasokukotskaja, and
F Antoni
October 1973,
Immunology,
