-transparent.png){kind=logo}
The proton NMR analysis of D-glucosaminate dehydratase reaction in D2O revealed the incorporation of a deuterium atom at C-3 carbon of the product, 2-keto-3-deoxy-D-gluconate. Based on the chemical shift of C-3 proton of the product and the coupling constant characteristic for the C-3 and C-4 axial-axial coupling in the 2C5 pyranose conformation, the deuterium is in the pro-S position. Thus, the dehydration of D-glucosaminate by the enzyme proceeds in a retention mode at C-3 carbon. Kinetic parameters show that the rate-determining step is the abstraction of alpha-proton from the substrate.
Related Publications
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
April 2003,
Biochimica et biophysica acta,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
March 1995,
Bioscience, biotechnology, and biochemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
January 1993,
Bioscience, biotechnology, and biochemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
April 1990,
Carbohydrate research,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
January 2005,
The Journal of biological chemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
March 2000,
Biochemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
May 1979,
The Journal of biological chemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
March 1962,
Biochemistry,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
May 2009,
Biotechnology letters,
R Iwamoto, and
Y Imanaga, and
S Sawada, and
K Soda
January 1980,
Analytical biochemistry,
