Biomaterial Database

Intracellular pH topography: determination by a fluorescent probe. 1983

J Slavík

The distribution of pH inside the yeast Endomyces magnusii was measured at 1 micron resolution using different external pH values. In a neutral buffer the pH of the cytoplasm was 6.7-7.2 in the center, decreasing to 6.0 toward the periphery of the cell. A decrease of external pH was followed by a gradual uniform decrease of internal pH. Using a comparison of the 'pH map' with phase-contrast picture of the same cell, the pH of the vacuoles was estimated to be 5.5-5.6.

UI	MeSH Term	Description	Entries
D002477	Cells	The fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.	Cell
D004718	Saccharomycetales	An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.	Budding Yeast,Endomycetales,Endomycopsis,Yeast, Budding,Budding Yeasts,Endomycetale,Endomycopses,Saccharomycetale,Yeasts, Budding
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence