BIOMAT Database Logo BIOMAT Database Logo

Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen. 1983

T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley

An enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to Salmonella typhi Vi antigen in human serum, and the results were compared with those from a previously described hemagglutination assay (HA). The ELISA detected Vi antibodies at a titer of greater than or equal to 20 in 40 (52%) of 77 sera from typhoid fever patients, whereas the HA gave titers of greater than or equal to 20 in 35 (47%). Determination of titers of serum specimens from 170 persons without typhoid fever revealed Vi antibody titers of greater than or equal to 20 in 4 (2.3%) by the ELISA and 3 (1.7%) by the HA. Unlike the sensitized erythrocytes used in the HA, the ELISA reagents have a shelf life of greater than or equal to 1 year. The ELISA may be preferred by some laboratories, especially those already performing other ELISA tests.

UI MeSH Term Description Entries
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D012485 Salmonella typhi A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER. Salmonella enterica serovar Typhi,Salmonella typhosa
D014435 Typhoid Fever An acute systemic febrile infection caused by SALMONELLA TYPHI, a serotype of SALMONELLA ENTERICA. Enteric Fever,Typhus, Abdominal,Salmonella typhi Infection,Typhoid,Abdominal Typhus,Enteric Fevers,Fever, Enteric,Fever, Typhoid,Fevers, Enteric,Fevers, Typhoid,Infection, Salmonella typhi,Infections, Salmonella typhi,Salmonella typhi Infections,Typhoid Fevers,Typhoids

Related Publications

T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Evaluation of enzyme-linked immunosorbent assay for detection of Salmonella typhi antigen and antibodies.
January 1991, The Journal of the Egyptian Public Health Association,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen.
February 1987, Journal of clinical microbiology,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
December 1987, Journal of clinical microbiology,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Comparison of diffusion-in-gel enzyme-linked immunosorbent assay with conventional serological methods for detection of class-specific antibodies to Salmonella typhi O antigen.
November 1980, Journal of clinical microbiology,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
[Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Salmonella Typhi lipopolysaccharide O and capsular polysaccharide Vi antigens in persons from outbreak of typhoid fever].
January 2015, Medycyna doswiadczalna i mikrobiologia,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies to Anaplasma marginale.
November 1988, Tropical animal health and production,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Enzyme-linked immunosorbent assay for brucella antigen detection in human sera.
February 1998, Journal of medical microbiology,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Enzyme-linked immunosorbent assay for Salmonella serology using lipopolysaccharide antigen.
October 1995, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies.
October 1984, Journal of clinical microbiology,
T J Barrett, and P A Blake, and S L Brown, and K Hoffman, and J M Llort, and J C Feeley
A novel enzyme-linked immunosorbent assay method for the detection of human neutrophil antigen-2a antibodies.
September 2009, Transfusion,
Copied contents to your clipboard!