Biomaterial Database

Purification and characterization of two distinct isozymes of protein carboxymethylase from bovine brain. 1983

D W Aswad, and E A Deight

Protein carboxymethylase (EC 2.1.1.24) from cytosol of bovine brain was found to exist as two apparent isozymes that could be separated by chromatography on DEAE-cellulose at pH 8.0. Rechromatography of the two forms, designated PCM I and PCM II, indicated that they are not interconvertible. Both enzymes have a molecular weight of 24,300 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, PCM I consists mainly of one isoelectric form, pI 6.5, whereas PCM II resolves into two forms of pI 5.6 and 5.7. The relative amounts of PCM I and PCM II show a marked tissue dependence. Brain has approximately twice as much PCM I as II, whereas liver contains only the type II enzyme. The two enzymes were found to have similar substrate specificities when tested with five different methyl-accepting proteins. Synapsin I, a basic protein associated with synaptic vesicles, was found to be an excellent methyl-accepting protein with regard to its Km (1.2 microM), but it exhibited a low stoichiometry of methyl incorporation.

UI	MeSH Term	Description	Entries
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011496	Protein Methyltransferases	Enzymes that catalyze the methylation of amino acids after their incorporation into a polypeptide chain. S-Adenosyl-L-methionine acts as the methylating agent. EC 2.1.1.	Protein Methylase,Protein Methylases,Protein Methyltransferase,Methylase, Protein,Methylases, Protein,Methyltransferase, Protein,Methyltransferases, Protein
D011497	Protein O-Methyltransferase	An enzyme that catalyzes the transfer of methyl groups from S-adenosylmethionine to free carboxyl groups of a protein molecule forming methyl esters. EC 2.1.1.-.	Protein Carboxylmethyltransferase,Protein Methylase II,Protein Methyltransferase II,Protein Carboxyl-Methylase,Protein Carboxymethylase,Protein O-Carboxymethyltransferase,Protein-Glutamic(Aspartic)-Methyltransferase,S-Adenosylmethionine Protein Carboxymethyltransferase,S-Adenosylmethionine Protein O-Methyltransferase,S-Adenosylmethionine-Protein Carboxymethyl Transferase,Carboxyl-Methylase, Protein,Carboxylmethyltransferase, Protein,Carboxymethyl Transferase, S-Adenosylmethionine-Protein,Carboxymethylase, Protein,Carboxymethyltransferase, S-Adenosylmethionine Protein,Methylase II, Protein,Methyltransferase II, Protein,O-Carboxymethyltransferase, Protein,O-Methyltransferase, Protein,O-Methyltransferase, S-Adenosylmethionine Protein,Protein Carboxyl Methylase,Protein Carboxymethyltransferase, S-Adenosylmethionine,Protein O Carboxymethyltransferase,Protein O Methyltransferase,Protein O-Methyltransferase, S-Adenosylmethionine,S Adenosylmethionine Protein Carboxymethyl Transferase,S Adenosylmethionine Protein Carboxymethyltransferase,S Adenosylmethionine Protein O Methyltransferase,Transferase, S-Adenosylmethionine-Protein Carboxymethyl
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002540	Cerebral Cortex	The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulci. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.	Allocortex,Archipallium,Cortex Cerebri,Cortical Plate,Paleocortex,Periallocortex,Allocortices,Archipalliums,Cerebral Cortices,Cortex Cerebrus,Cortex, Cerebral,Cortical Plates,Paleocortices,Periallocortices,Plate, Cortical
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia