Evaluation of slide agglutination methods for identifying group D streptococci. 1983

G T Chang, and P D Ellner

Three commercial reagents for the rapid identification of group D streptococci by slide agglutination were evaluated. These included SeroSTAT (Scott Laboratories, Fiskeville, R.I.), Streptex (Wellcome Laboratories, Research Triangle Park, N.C.), and Phadebact (Pharmacia Diagnostics, Piscataway, N.J.). The methods included direct colony testing, enzyme extraction with pronase, and broth culture. A total of 72 strains of group D streptococci were tested. The SeroSTAT and Streptex reagents with pronase extraction each identified 65 (90%) of the strains. The SeroSTAT reagent was somewhat more specific since it did not cross-react with other streptococci of the viridans group. The Phadebact reagent was nonreactive. We conclude that the latex reagents can be very useful for the quick recognition of group D streptococci in the clinical laboratory.

UI MeSH Term Description Entries
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination
D013291 Streptococcus A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.

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