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Suspension cultures of Chinese hamster ovary (CHO) cells were exposed to methyl methanesulfonate (MMS) or methylnitrosourea (MNU) and assayed for mutation induction (6-thioguanine resistance) and for specific DNA adducts. DNA methylation at the 1-, 3- and 7-positions of adenine, the 3-, O6- and 7-positions of guanine, and phosphate was detected in cultures exposed to MMS, while MNU produced 3- and 7-methyladenine, 3-methylcytosine, 3-, O6- and 7-methylguanine, O4-methylthymidine and methylated phosphodiesters. When mutations induced by MMS and MNU were compared by linear correlation analysis with levels of each of these adducts, only O6-methylguanine displayed a strong correlation with mutations (r = 0.879, p less than 0.001). The relationship between O6-methylguanine and induced mutations in CHO cells is similar to that previously reported in CHO cells for O6-ethylguanine and mutations (Heflich et al., 1982) and indicates that alkylation-induced mutations at the HGPRT locus in CHO cells are primarily associated with O6-alkylguanine formation.
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
August 1979,
Chemico-biological interactions,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
September 1982,
Mutation research,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
January 1984,
Basic life sciences,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
January 1998,
Mutagenesis,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
October 1977,
Mutation research,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
June 1983,
Molecular and cellular biology,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
October 1977,
Mutation research,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
January 1986,
Progress in clinical and biological research,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
January 1982,
Mutation research,
D T Beranek, and
R H Heflich, and
R L Kodell, and
S M Morris, and
D A Casciano
January 1984,
Teratogenesis, carcinogenesis, and mutagenesis,
