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The uptake of dimethialium, a thiamine analog having a methyl group in place of the hydroxyethyl group in the thiazole moiety, was studied in freshly isolated rat hepatocytes. In an Na+-medium, dimethialium at 10 microM was accumulated rapidly by the cells and an almost steady intra- to extracellular distribution ratio of 4.2 was attained in 5 min of incubation. The Kt and the Vmax for the saturable component were estimated to be 27 microM and 19 pmol/10(5) cells per min, respectively. In a K+ medium, the uptake of dimethialium was decreased to 58% of that of control. Ouabain and 2,4-dinitrophenol significantly lowered the rate of dimethialium uptake. Both phenylthiazinothiamine and oxythiamine were inhibitory on the uptake of dimethialium, which uptake was also inhibited by choline. These data indicate that dimethialium transport in liver cells proceeds via a carrier-mediated active process dependent on Na+ and biological energy. Furthermore, these results also suggest that thiamine transport in liver is dissociable from thiamine phosphorylation.
K Yoshioka, and
H Nishimura, and
A Iwashima
April 1980,
Experientia,
K Yoshioka, and
H Nishimura, and
A Iwashima
January 1978,
Journal of nutritional science and vitaminology,
K Yoshioka, and
H Nishimura, and
A Iwashima
July 1974,
FEBS letters,
K Yoshioka, and
H Nishimura, and
A Iwashima
January 1982,
The Journal of membrane biology,
K Yoshioka, and
H Nishimura, and
A Iwashima
January 1983,
Advances in prostaglandin, thromboxane, and leukotriene research,
K Yoshioka, and
H Nishimura, and
A Iwashima
July 1981,
Biochemical pharmacology,
K Yoshioka, and
H Nishimura, and
A Iwashima
January 1989,
Drug metabolism and disposition: the biological fate of chemicals,
K Yoshioka, and
H Nishimura, and
A Iwashima
February 1983,
The American journal of physiology,
K Yoshioka, and
H Nishimura, and
A Iwashima
December 1981,
Japanese journal of pharmacology,
K Yoshioka, and
H Nishimura, and
A Iwashima
August 1979,
The Journal of biological chemistry,
