Biomaterial Database

Absence of circulating immune complexes containing DNA in hemodialysed sera. 1983

M K Dasgupta, and P Davis, and K V Johny, and M R Higgins, and J B Dossetor

Although the presence of free DNA and antibodies to DNA in the sera of patients on maintenance hemodialysis has been reported, we found only a low incidence of antibodies to single-stranded DNA (ss-DNA) and native DNA (n-DNA) using an improved detection technique. In our search for evidence of the possible formation of DNA-anti-DNA circulating immune complexes (CIC), antibodies to n-DNA were detected in the sera of only 2 of 53 dialysed and 1 of 25 non-dialysed renal patients. CICs were detected in the sera of 17 of 53 dialysed (28% by Raji-RIA, 6% by Clq-BA) and 6 of 25 non-dialysed (20% by Raji-RIA and 24% by Clq-BA) renal patients: the difference is not significant (P greater than 0.25). DNase digestion of the dialysed sera that were positive for CIC by Raji-RIA produced no significant change in the results on re-assay (P greater than 0.50) in contrast with a significant decrease in CIC after DNase digestion of selected SLE sera and of solutions containing DNA-anti-DNA complexes formed in vitro (P less than 0.05). The formation of circulating DNA-anti-DNA complexes appears not to be the cause of the low incidence of antibodies to n-DNA in the sera of dialysed patients.

UI	MeSH Term	Description	Entries
D007676	Kidney Failure, Chronic	The end-stage of CHRONIC RENAL INSUFFICIENCY. It is characterized by the severe irreversible kidney damage (as measured by the level of PROTEINURIA) and the reduction in GLOMERULAR FILTRATION RATE to less than 15 ml per min (Kidney Foundation: Kidney Disease Outcome Quality Initiative, 2002). These patients generally require HEMODIALYSIS or KIDNEY TRANSPLANTATION.	ESRD,End-Stage Renal Disease,Renal Disease, End-Stage,Renal Failure, Chronic,Renal Failure, End-Stage,Chronic Kidney Failure,End-Stage Kidney Disease,Chronic Renal Failure,Disease, End-Stage Kidney,Disease, End-Stage Renal,End Stage Kidney Disease,End Stage Renal Disease,End-Stage Renal Failure,Kidney Disease, End-Stage,Renal Disease, End Stage,Renal Failure, End Stage
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D006435	Renal Dialysis	Therapy for the insufficient cleansing of the BLOOD by the kidneys based on dialysis and including hemodialysis, PERITONEAL DIALYSIS, and HEMODIAFILTRATION.	Dialysis, Extracorporeal,Dialysis, Renal,Extracorporeal Dialysis,Hemodialysis,Dialyses, Extracorporeal,Dialyses, Renal,Extracorporeal Dialyses,Hemodialyses,Renal Dialyses
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000936	Antigen-Antibody Complex	The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.	Immune Complex,Antigen-Antibody Complexes,Immune Complexes,Antigen Antibody Complex,Antigen Antibody Complexes,Complex, Antigen-Antibody,Complex, Immune,Complexes, Antigen-Antibody,Complexes, Immune