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The Eco RI fragment "b" of chicken DNA (Breathnach, Mandel and Chambon, 1977), which contains the sequences coding for the 5' quarter of ovalbumin mRNA (ov mRNA), has been isolated by molecular cloning using a "shotgun" approach. Electron microscopy and restriction enzyme analysis have revealed that the sequences coding for the 5' quarter (approximately 500 nucleotides) of ov mRNA are split into four regions separated by three intervening sequences. The cloning procedure seems to be reliable, since the restriction enzyme pattern of the cloned Econ RI fragment "b" is similar to that of the corresponding chromosomal DNA fragment. There is no evidence supporting the existence of a 150-200 nucleotide long sequence at the 5' end of the ov mRNA similar to the "leader" sequences found at the 5' end of some adenovirus and SV40 mRNAs.
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B Cami, and
W Roskam, and
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J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
July 1978,
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B Cami, and
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J P Le Pennec, and
F Perrin, and
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M Cochet, and
P Chambon
January 1979,
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B Cami, and
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J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
July 1978,
Nature,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
November 1977,
Nature,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
January 1979,
Proceedings of the National Academy of Sciences of the United States of America,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
March 1977,
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A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
June 1982,
Nature,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
October 1978,
Cell,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
January 1979,
Cell,
A C Garapin, and
B Cami, and
W Roskam, and
P Kourilsky, and
J P Le Pennec, and
F Perrin, and
P Gerlinger, and
M Cochet, and
P Chambon
April 1979,
Science (New York, N.Y.),
