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The cytotoxicity of fractions, subfractions, and some individual components present in tar from a low-Btu coal gasifier was determined in vitro with cultured dog alveolar macrophages. A subfraction of the tar which contained phenols and neutral nitrogen heterocycles was found to be a major contributor to the cytotoxic activity of the tar. Four compounds present in this subfraction were tested individually. Ranked from the most to the least toxic they were: 9-phenanthrol, 2-fluorenol, phenanthridone, and carbazole.
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