-transparent.png){kind=logo}
A convenient, accurate assay was developed for determining skin cholesta-5,7-dien-3 beta-ol (7,8-didehydrocholesterol) concentrations. Ultraviolet spectrophotometry provided quantitation of the sterol from rat skins following saponification and chromatography on Lipidex and high-performance liquid chromatography. Correction for recoveries was accomplished by using 7,8-didehydro[3 alpha-3H]cholesterol as an internal standard. Chronic dosing of vitamin D-deficient rats with 1,25-dihydroxyvitamin D3 caused a 4-fold increase in skin 7-dehydrocholesterol content. This rise was not the result of changes in food consumption, body weight, or plasma calcium. Cholesterol concentrations were not significantly elevated although some of the other nonsaponifiable lipid components found in the high-performance liquid chromatogram appeared to be increased by the treatment. These results suggest that the vitamin D hormone 1,25-(OH)2D3 may exert a positive feedback regulation on the production of vitamin D3 in skin.
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
December 1986,
Archives of biochemistry and biophysics,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
November 1981,
Biochemical and biophysical research communications,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
September 1983,
The Biochemical journal,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
September 1986,
Nutrition reviews,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
August 1984,
Toxicology and applied pharmacology,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
December 1989,
Proceedings of the National Academy of Sciences of the United States of America,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
October 1979,
Biochemical and biophysical research communications,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
October 1980,
Proceedings of the National Academy of Sciences of the United States of America,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
May 1994,
The Journal of investigative dermatology,
R P Esvelt, and
H F DeLuca, and
J K Wichmann, and
S Yoshizawa, and
J Zurcher, and
M Sar, and
W E Stumpf
June 1985,
The American journal of physiology,
