Biomaterial Database

Partition of parinaroyl phospholipid probes between solid and fluid phosphatidylcholine phases. 1982

R Welti, and D F Silbert

The partitioning of parinaroyl phospholipid probes between solid- and fluid-phase phospholipid is examined. The immiscible model system dipalmitoylphosphatidylcholine and palmitoyldocosahexaenoylphosphatidylcholine is used. Fluorescence quantum yields and fluorescence polarization are used to calculate Kps/f, the solid to fluid phase partition coefficient of each probe [Sklar, L. A., Miljanich, G. P. & Dratz, E. A. (1979) Biochemistry 18, 1707-1716]. Like free trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid) 1-palmitoyl-2-trans-parinaroylphosphatidylcholine and 1-palmitoyl-2-tra-parinaroylphosphatidylethanolamine partition strongly into solid phase with mean Kps/fs of 7 +/- 4 and 9 +/- 5, respectively. Like free cis-parinaric acid (9,11,13,15-cis-trans,trans,cis-octadecatetraenoic acid), another group of phospholipid probes prefer fluid phase just slightly or partition equally between fluid and solid phases. The Kps/f of 1-palmitoyl-2-cis-parinaroylphosphatidylcholine is 0.6 +/- 0.2, that of 1-palmitoyl-2-cis-parinaroylphosphatidylethanolamine is 1.0 +/- 0.3, that of 1-oleoyl-2-trans-parinaroylphosphatidylcholine is 0.6 +/- 0.3, and that of 1-oleoyl-2-trans-parinaroylphosphatidylethanolamine is 0.7 +/- 0.3. Two probes partition more strongly into fluid-phase phospholipid. These are 1-oleoyl-2-cis-parinaroylphosphatidylcholine with a Kps/f of 0.2 +/- 0.1 and 1-oleoyl-2-cis-parinaroylphosphatidylethanolamine with a Kps/f of 0.4 +/- 0.4.

UI	MeSH Term	Description	Entries
D008433	Mathematics	The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Mathematic
D008560	Membrane Fluidity	The motion of phospholipid molecules within the lipid bilayer, dependent on the classes of phospholipids present, their fatty acid composition and degree of unsaturation of the acyl chains, the cholesterol concentration, and temperature.	Bilayer Fluidity,Bilayer Fluidities,Fluidities, Bilayer,Fluidities, Membrane,Fluidity, Bilayer,Fluidity, Membrane,Membrane Fluidities
D008563	Membrane Lipids	Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.	Cell Membrane Lipid,Cell Membrane Lipids,Membrane Lipid,Lipid, Cell Membrane,Lipid, Membrane,Lipids, Cell Membrane,Lipids, Membrane,Membrane Lipid, Cell,Membrane Lipids, Cell
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D011663	Pulmonary Surfactants	Substances and drugs that lower the SURFACE TENSION of the mucoid layer lining the PULMONARY ALVEOLI.	Surfactants, Pulmonary,Pulmonary Surfactant,Surfactant, Pulmonary
D005231	Fatty Acids, Unsaturated	FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.	Fatty Acids, Polyunsaturated,Polyunsaturated Fatty Acid,Unsaturated Fatty Acid,Polyunsaturated Fatty Acids,Acid, Polyunsaturated Fatty,Acid, Unsaturated Fatty,Acids, Polyunsaturated Fatty,Acids, Unsaturated Fatty,Fatty Acid, Polyunsaturated,Fatty Acid, Unsaturated,Unsaturated Fatty Acids
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry