BIOMAT Database Logo BIOMAT Database Logo

Activation of the alternative complement pathway by extracts of cotton dust. 1980

M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer

Extracts of cotton dust were tested for their ability to activate the alternative complement pathway in fresh normal human serum (NHS). Alternative pathway activation was determined by a haemolytic assay utilizing glutathione-sensitized human erythrocytes, consumption of alternative pathway components in terms of alternative pathway CH50 units and an immunoelectrophoretic assay to detect split products of activation of factor B. All assays were performed under conditions that have been shown to block the initial steps of classical pathway activation but permit activation of the alternative complement pathway. Results demonstrate that the cotton dust extracts could consume alternative complement pathway proteins in a dose-response manner. The complement activating factor is probably endotoxin since a cotton dust extract obtained by an extraction method for endotoxin yielded the greatest activity.

UI MeSH Term Description Entries
D011415 Complement Factor B A glycine-rich, heat-labile serum glycoprotein that contains a component of the C3 CONVERTASE ALTERNATE PATHWAY (C3bBb). Bb, a serine protease, is generated when factor B is cleaved by COMPLEMENT FACTOR D into Ba and Bb. C3 Proactivator,C3PA,Complement 3 Proactivator,Factor B,Properdin Factor B,Bb Fragment of Factor B,Complement Factor B Fragment, Bb,Complement Factor B, Alternative Pathway,Complement Factor B-Derived Fragment Bb,Complement Factor Ba,Complement Factor Bb,Complement Protein B,Complement Protein Factor B,Properdin Factor Ba,Properdin Factor Bb,Properdin Factor Bf,Properdin Factor Bf F1,Bb, Complement Factor,Complement Factor B Derived Fragment Bb,Factor B, Complement,Factor B, Properdin,Factor Ba, Complement,Factor Ba, Properdin,Factor Bb, Complement,Factor Bb, Properdin,Factor Bf, Properdin,Proactivator, C3,Proactivator, Complement 3,Protein B, Complement
D003167 Complement Activation The sequential activation of serum COMPLEMENT PROTEINS to create the COMPLEMENT MEMBRANE ATTACK COMPLEX. Factors initiating complement activation include ANTIGEN-ANTIBODY COMPLEXES, microbial ANTIGENS, or cell surface POLYSACCHARIDES. Activation, Complement,Activations, Complement,Complement Activations
D003170 Complement Pathway, Alternative Complement activation initiated by the interaction of microbial ANTIGENS with COMPLEMENT C3B. When COMPLEMENT FACTOR B binds to the membrane-bound C3b, COMPLEMENT FACTOR D cleaves it to form alternative C3 CONVERTASE (C3BBB) which, stabilized by COMPLEMENT FACTOR P, is able to cleave multiple COMPLEMENT C3 to form alternative C5 CONVERTASE (C3BBB3B) leading to cleavage of COMPLEMENT C5 and the assembly of COMPLEMENT MEMBRANE ATTACK COMPLEX. Alternative Complement Pathway,Properdin Pathway,Alternative Complement Activation Pathway,Complement Activation Pathway, Alternative
D003368 Gossypium A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES. Cotton Plant,Cotton Plants,Gossypiums,Plant, Cotton,Plants, Cotton
D004391 Dust Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed) House Dust,Housedust,Dust, House
D006461 Hemolysis The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity. Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Antibody-independent complement activation by cardroom cotton dust.
August 1979, Environmental research,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
In vitro activation of the alternative pathway of complement by settled grain dust.
November 1978, The Journal of allergy and clinical immunology,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
In vitro alternative and classical activation of complement by extracts of cotton mill dust: a possible mechanism in the pathogenesis of byssinosis.
October 1983, Environmental research,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
The alternative pathway of complement activation.
January 1976, Advances in immunology,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Activation of the alternative complement pathway.
November 1979, CRC Critical reviews in immunology,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Alternative complement pathway activation by C4b deposited during classical pathway activation.
April 1986, Journal of immunology (Baltimore, Md. : 1950),
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Activation of the alternative complement pathway by Histoplasma capsulatum.
October 1980, Infection and immunity,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Activation of the alternative complement pathway by Naegleria fowleri.
October 1980, Infection and immunity,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Activation of the alternative complement pathway by fungal melanins.
January 2002, Clinical and diagnostic laboratory immunology,
M R Wilson, and A Sekul, and R Ory, and J E Salvaggio, and S B Lehrer
Activation of the alternative complement pathway by intraocular lenses.
June 1985, Investigative ophthalmology & visual science,
Copied contents to your clipboard!