Biomaterial Database

[alpha-n-benzoylarginine-2-naphthylamide-amidohydrolase activity in chicken liver (author's transl)]. 1980

F Barceló, and N Vives, and J Bozal

The enzyme activity from chicken liver that hydrolizes alpha-N-benzoil-DL-arginine-2-naphthylamide (BANA) has been separated into three active fractions by chromatography on Sephadex G-100 and DEAE-cellulose. The enzyme in Fraction FEA has a molecular weight greater than or equal to 100,000. The other two enzymes in Fractions FE1B and FE2B have a molecular weight around 23,000 and are separated by DEAE-cellulose chromatography. The BANA-hydrolase of fraction FE2B is unstable at pH alkaline and activates trypsinogen, in contrast with the enzyme of fraction FEB1. Both are thiol enzymes which behave analogously against activators (cysteine, dithiotreitol and 2-mercaptoethanol) and inhibitors (PCMB, IAA, N-ethylmaleimide, Cu2+, Hg2+ and Zn2+).

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D002403	Cathepsins	A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.	Cathepsin
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003546	Cysteine Endopeptidases	ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D000626	Aminopeptidases	A subclass of EXOPEPTIDASES that act on the free N terminus end of a polypeptide liberating a single amino acid residue. EC 3.4.11.	Aminopeptidase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001587	Benzoylarginine-2-Naphthylamide	An enzyme substrate which permits the measurement of peptide hydrolase activity, e.g. trypsin and thrombin. The enzymes liberate 2-naphthylamine, which is measured by colorimetric procedures.	Benzoylarginine beta-Naphthylamide,Benzoylarginine 2 Naphthylamide,2 Naphthylamide, Benzoylarginine,Benzoylarginine beta Naphthylamide,Naphthylamide, Benzoylarginine 2,beta-Naphthylamide, Benzoylarginine
D056655	Cathepsin H	An ubiquitously-expressed lysosomal cysteine protease that is involved in protein processing. The enzyme has both endopeptidase and aminopeptidase activities.