Biomaterial Database

Synthesis of transfer ribonucleic acid in KB cells infected with adenovirus type 2. 1978

L M Sehulster, and F Varricchio, and Raska K Jun

The synthesis of low mol. wt. RNA has been studied in KB cells infected with adenovirus type 2 after labelling with 3H-uridine or 32P. An increasing amount of virus-coded VA RNA is detected from 8 h after infection onward. The rate of synthesis of tRNA is unchanged up to 16 h after infection and thereafter decreases; from 24 to 48 h after infection, the specific activity of tRNA is about 60% of the control value. The specific activity of cellular 5S RNA increases from 12 h after infection. When the tRNA is analysed by a two-dimensional gel electrophoresis system resolving the tRNA into 42 to 47 spots, changes in synthesis of tRNA in individual spots are seen. From 8 to 12 h after infection, an increase in the relative rate of incorporation into RNA is observed in 7 spots, while a significant decrease is detected in 8 spots. From 12 to 16 h after infection, incorporation into RNA is increased in 6 spots, but is most marked (sevenfold) in 1 spot. A decrease of incorporation into RNA in 6 spots is observed at the same time. From 24 to 34 h after infection, an increase in synthesis of RNA in 8 spots is observed and a decrease also in 8 spots. Incorporation into RNA in 2 spots is virtually shut off.

UI	MeSH Term	Description	Entries
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D000260	Adenoviruses, Human	Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-G.	APC Viruses,APC Virus,Adenovirus, Human,Human Adenovirus,Human Adenoviruses
D012334	RNA, Neoplasm	RNA present in neoplastic tissue.	Neoplasm RNA
D012343	RNA, Transfer	The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.	Suppressor Transfer RNA,Transfer RNA,tRNA,RNA, Transfer, Suppressor,Transfer RNA, Suppressor,RNA, Suppressor Transfer
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D014779	Virus Replication	The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.	Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications