Biomaterial Database

Factors influencing detection of tolerance in Staphylococcus aureus. 1982

W H Goessens, and P Fontijne, and M F Michel

The phenomenon of tolerance to cloxacillin and methicillin was studied in Staphylococcus aureus. It was demonstrated that the minimal bactericidal concentrations showed marked differences, depending on the method of detection used. These differences resulted from carry-over of the antibiotic to the subculture plates. When carry-over of the antibiotic was prevented by the addition of beta-lactamase to the nutrient medium, the antibiotics were no longer bactericidal. At a certain antibiotic concentration and at higher concentrations, however, each strain showed a certain survival percentage after 24 h. The tolerance percentage was determined for 15 strains. The values found for the individual strains ranged from less than 0.1 to 11% for cloxacillin and methicillin. since these percentages were reproducible within narrow limits, they could be regarded as a characteristic of the strains. The tolerance percentage was independent of the growth phase of the initial cultures.

UI	MeSH Term	Description	Entries
D008712	Methicillin	One of the PENICILLINS which is resistant to PENICILLINASE but susceptible to a penicillin-binding protein. It is inactivated by gastric acid so administered by injection.	Penicillin, Dimethoxyphenyl,Methicillin Hydrate, Monosodium Salt,Methicillin Monohydrate, Monosodium Salt,Methicillin Sodium,Meticillin,Metin,Staphcillin,Dimethoxyphenyl Penicillin
D010403	Penicillin Resistance	Nonsusceptibility of an organism to the action of penicillins.	Penicillin Resistances,Resistance, Penicillin,Resistances, Penicillin
D003023	Cloxacillin	A semi-synthetic antibiotic that is a chlorinated derivative of OXACILLIN.	Chloroxacillin,Sodium Cloxacillin,Cloxacillin Sodium,Syntarpen,Tegopen,Cloxacillin, Sodium,Sodium, Cloxacillin
D000900	Anti-Bacterial Agents	Substances that inhibit the growth or reproduction of BACTERIA.	Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.